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HiScanSQ system for next generation sequencing and microarray-based analyses

HiScanSQ; Next generation sequencing; Exome sequencing; Gene panels; Microarray analysis; CGH; CNV.

HiScanSQ system for next generation sequencing and microarray-based analyses

Contacts and Location

Contact 1 Zanda Daneberga
Contact 2 Linda Gabrusenoka
Enquire about this equipment
Organisational Unit Rīga Stradiņš University
    Institute of Oncology - Latvia, Rīga

Description

HiScanSQ; Next generation sequencing; Exome sequencing; Gene panels; Microarray analysis; CGH; CNV.

Specification

For NGS TruSeqv3 chemistry (paired-end sequencing, coupled with 2x100 bp read length). Troughput: up to 17,5 Gb per day for a 2x100 bp run. Performance greater as 80% higher as Q30 at 2x100bp. Microarry BeadArray technology is based on 3-micron silica beads that self assemble in microwells on either of two substrates: fiber optic bundles or planar silica slides. When randomly assembled on one of these two substrates, the beads have a uniform spacing of 5.7 microns. Each bead is covered with hundreds of thousands of copies of a specific oligonucleotide that act as the capture sequences in a given Illumina assay. The BeadChip array format is used in Illumina's Infinium Genotyping, DASL Gene Expression, and focused genotyping arrays.

Services

The HiScanSQ system integrates the power and resolution of next-generation sequencing with the high-throughput capacity of genotyping and gene expression arrays, delivering unprecedented flexibility for experimental design. The instrument features two distinct components, the HiScan Reader and the SQ Module. The HiScan Reader functions as a high-speed, precision imaging scanner for Illumina sequencing and microarray-based analyses. The SQ Module is reagent handling fluidics device needed to perform Illumina next-generation sequencing. The integration of sequencing and microarray-based experiments offers unlimited possibilities for genomic research. Illumina microarrays are ideal for large-scale population study researchers and biobanks performing genome-wide and phenome-wide association studies (GWAS and PheWAS). They also offer the high resolution required for detection of copy number variant (CNV), loss of heterozygosity (LOH), and insertions/deletions.

Status

Engaged in service

Funding Source

Other -
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Manufacturer

Illumina (manufacturer's website)

Model

HiScanSQ

Availability

Information on availability on request

Access Level

Full Access

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