Inverted Widefield Fluorescence Microscope with FRET Module

The Eclipse Ti-U inverted research microscope con- tains Nikon’s CFI60 optical system and a wide choice of objectives for bright field, dark field, fluorescence, phase contrast, DIC, and modulation contrast microscopy techniques. The microscope is equipped with two Andor iXon Ultra EMCCD cameras allowing FRET measurements. Additional equipment: Integrated CO2 incubator with temperature setting.

• Port: 3 ports: Eyepiece 100%, left 100%, right 100%, AUX
• Focusing: Via nosepiece up/down movement; Stroke (manual): up 8mm, down 3mm; Coarse stroke:5.0mm/rotation; Fine stroke: 0.1mm/rotation; Minimum fine reading: 1µm; Coarse refocusing mechanism
• Intermediate magnification: 1.5x
• Eyepiece tube body: TI-TD Binocular Tube D, TI-TS Binocular Tube S, TI-TERG Ergonomic Tube
• Eyepiece tube base: TI-T-B Eyepiece Tube Base Unit, TI-T-BPH Eyepiece Tube Base Unit for PH, TI-T-BS Eyepiece Tube Base Unit with Side Port
• Eyepiece lens: CFI 10x, 12.5x, 15x
• Illumination pillar: TI-DS Diascopic Illumination Pillar 30W, TI-DH Diascopic Illumination Pillar 100W
• Condenser: ELWD condenser, LWD condenser, NAMC condenser, ELWD-S condenser, High NA condenser, Darkfield condenser, CLWD condenser; TI-ND6-E Motorized Sextuple DIC Nosepiece, TI-N6 Sextuple Nosepiece, TI-ND6 Sextuple DIC Nosepiece
• Objectives: CFI60 objectives
• Epi-fluorescence attachment: Sextuple fluorescence filter cube rotating turret, Filter cubes with noise terminator mechanism, Field diaphragm centerable, 33mm ND4/ND8 filters, 25mm heat absorbing filter
• Nomarski DIC system: Contrast control: Senarmont method (by rotating polarizer); Condenser side prism: LWD N1/N2/NR (Dry), HNA N2/NR (Dry/Oil) types
• Fluorescence illumination system: Lambda DG-4 (Sutter Instrument Company)
• Detector cameras: Two iXon Ultra 897 EMCCD cameras (Andor)

Widefield fluorescence or bright field microscopy in slide preparations or cell culture vessels for:

• FRET measurements in live or fixed cells for studying protein-protein interactions and protein conformational changes
• Immunocytochemistry or immunohistochemistry preparations
• Immunofluorescence assays
• Histological preparations (morphology)