Share this
Map

Confocal Laser Scanning Microscope

Capturing high-quality confocal images at ultrahigh- speed and enhanced sensitivity with a resonant scanner and galvano scanner, A1R+ is a powerful tool for the acquisition of intracellular dynamics and interaction. A1R+ has a hybrid scan

Confocal Laser Scanning Microscope

Contacts and Location

Contact 1 Mārtiņš Kālis
Contact 2 Linda Gabrusenoka
Enquire about this equipment
Organisational Unit Rīga Stradiņš University
    Institute of Microbiology and Virology - Latvia, Rīga

Description

Capturing high-quality confocal images at ultrahigh-speed and enhanced sensitivity with a resonant scanner and galvano scanner, A1R+ is a powerful tool for the acquisition of intracellular dynamics and interaction. A1R+ has a hybrid scanner head that incorporates both an ultrahigh-speed resonant scanner and a high-resolution galvano scanner. Simultaneous photoactivation and ultrafast imaging using these two scanners allow acquisition of rapid changes after photoactivation and enables observation of intermolecular interaction. Microscope can perform FRET applications. Additional equipment: Integrated CO2 incubator with temperature setting.

Specification

  • 4 lasers: 405 nm, 561/594 nm, 638/640 nm, Ar laser (457 nm, 476 nm, 488 nm, 514 nm)
  • Standard fluorescence detector: 400-750 nm
  • Filter cubes used for a microscope mountable on each of three filter wheels,wavelengths: 450/50, 525/50, 595/50, 700/75 nm
  • Standard image acquisition scanner: galvano scanner x 2
  • Pixel size: max. 4096 x 4096 pixels
  • Standard mode: 2 fps (512 x 512 pixels, bi-direction), 24 fps (512 x 32 pixels, bi-direction)
  • Fast mode: 10 fps (512 x 512 pixels, bi-direction), 130 fps (512 x 32 pixels, bi-direction)*1
  • Zoom: 1-1000x continuously variable
  • Scanning mode: X-Y, X-T, X-Z, XY rotation, Free line
  • High-speed image acquisition scanner: resonant scanner (X-axis, resonance frequency 7.8 kHz), galvano scanner (Y-axis)
  • Pixel size: max. 512 x 512 pixels
  • Scanning speed: 30 fps (512 x 512 pixels) to 420 fps (512 x 32 pixels), 15,600 lines/sec (line speed)
  • Zoom: 7 steps (1x, 1.5x, 2x, 3x, 4x, 6x, 8x)
  • Scanning mode: X-Y, X-T, X-Z
  • Acquisition method: Standard image acquisition, High-speed image acquisition, Simultaneous photoactivation and image acquisition
  • Spectral detector: 32 channels, Wavelength detection range 400-750 nm, Spectral image acquisition speed: 4 fps (256 x 256 pixels), 1000 lps
  • Pixel size: max. 2048 x 2048
  • Wawelength resolution: 80 nm (2.5 nm), 192 nm (6 nm), 320 nm (10 nm, Wavelength range variable in 0.25 nm steps
  • Software: 2D analysis, 3D volume rendering/orthogonal, 4D analysis, spectral unmixing
  • Application: FRAP, FLIP, FRET, photoactivation, three-dimensional time-lapse imaging, multipoint time-lapse imaging, colocalization
  • Additional equipment: Integrated CO2 incubator with temperature setting

Services

Confocal, widefield fluorescence or bright field microscopy in slide preparations or cell culture vessels for:  

  • Immunocytochemistry or immunohistochemistry preparations  
  • Immuno-fluorescence assays
  • Histological preparations(morphology)
  • 2D confocal microscopy imaging
  • 3D confocal imaging by optical sectioning  
  • Time-lapse imaging of 2D and 3D images  
  • Photo bleaching

Status

Upon agreement

Funding Source

Other -
Back to all
0 items in cart
Total: 0
You have no items in a cart
Go to checkout
Go Enquire