Spectrofluorometer FluoroMax-3, Jobin Yvon

FluoroMax®-3 is self-contained, fully automated spectrofluorometer system. Data output is viewed on a PC, while printouts may be obtained via an optional plotter or printer. All FluoroMax®-3 functions is under the control of DataMax spectroscopy software. The main parts of the Fluoro- Max®-3 spectrofluorometer system are: • State-of-the-art optical components • A personal computer • DataMax for Windows™, the driving software.

Excitation Source: 150-W xenon, continuous output, ozone-free lamp Optics: All-reflective, for focusing at all wavelengths and precise imaging for microsamples. Dispersion: 4.25 nm mm–1 Spectrometer: Single-grating excitation and emission spectrometer (standard). Spectrometer are f/3.5 Czerny-Turner design with classicallyruled gratings and all-reflective optics, using 1200-grooves/mm gratings: Resolution: 0.3 nm Maximum scan speed: 200 nm s–1 Accuracy: ±0.5 nm Step Size: 0.0625–100 nm Range: 0–950 nm (physical) Gratings: Excitation 330-nm blaze (220–600 nm optical range) Emission 500-nm blaze (290–850 nm optical range) Sample Module: The sample module also has a removable gap-bed assembly for sampling accessory replacement. Detectors: • Calibrated photodiode for excitation reference correction from 200–980 nm. • Emission detector is an R928P for high sensitivity in photoncounting mode (180–850 nm). High voltage = 950 V, linearity to 2 × 106 counts s–1, < 1000 dark counts s–1. Sensitivity: Double-distilled, de-ionized, ICP-grade water-Raman scan 2500:1 S/N at 397 nm, 5-nm bandpass, 1-s integration time, background noise first standard deviation at 450 nm. 300 000 counts s–1 using these conditions. Excitation shutter: Computer-controlled Integration time: 0.001–160 s Slit width: 0–30 nm bandpass, continuously adjustable via host computer

A spectrofluorometer is an analytical instrument used to measure and record the fluorescence of a sample. While recording the fluorescence, the excitation, emission, or both wavelengths may be scanned. With additional accessories, variation of signal with time, temperature, concentration, polarization, or other variables may be monitored.