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Gene expression studies

Real-time quantitative polymerase chain reaction. Expression analyses with relative quantification method (2-ΔΔCT).

Contacts and Location

Contact 1 Tatjana Sjakste
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Organisational Unit Institute of Biology
    University of Latvia

Description

Real-time quantitative polymerase chain reaction. Expression analyses with relative quantification method (2-ΔΔCT).

Specification

Tissues should be frozen in liquid nitrogen or preserved in a way that is suitable for RNA isolation. Blood should be taken in EDTA vacutainers. RNA extraction and cDNA preparation Total RNA was isolated from animal or human tissues or cell suspension. Different primers designing.

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