Microplate Reader

Scans 96-well or 384-well plates, 340-850nm wavelength range. Applications include immuno- assays, protein assays, growth curves and hormones, hybridisation assays, mini-sequencing assays, cytotoxicity, cell proliferation, cell adhesion, signal transduction, enzyme assays, endotoxins, antioxidants, and food diagnostics.

• Wavelength range:340-850 nm (With Appriopriate Filters)
• Plate Formats: 96- and 384-well plates
• Measurement Types: Single, Dual Wavelength, Kinetic, or Dual Kinetic
• Measurement Modes: Continous or Stepping
• Linearity (96-well plate): 0-4 Abs, +/-2% 0-3 Abs, +/-2%, 340 nm and 405 nm
• Linearity (384-well plate): 0-3 Abs, +/-2% 0-2 Abs, +/-2% with continuous mode
• Read-out range: 0-6 Abs
• Accuracy: +/-1% or 0.003 Abs, whichever is greater (0-2 Abs) +/-2% (2-3 Abs)
• Precision: CV<0.2% (0.3-3 Abs), CV<1.0% (3-4 Abs) stepping mode
• Measurement time (96-well plate): 9 s, continuous 14 s, stepping
• Measurement time (384-well plate): 14 s, continuous 42 s, stepping
• Shaking:Linear shaking, 3 speeds
• Filters: Eight-position filter wheel with 405, 450, 490 and 650nm Filters

• Protein-copper chelation and secondary detection of the reduced copper
• Protein-dye binding and direct detection of the colour change associated with the bound dye
• The enzyme-linked immunosorbent assay (ELISA) for quantitative and/or qualitative detection of antibodies and proteins
• Kinetic assays for measuring enzymatic activity
• Cell proliferation and cytotoxicity assays: ADP, ATP and ADP/ATP ratio assays, bioluminescence cytotoxicity assays, based on measurement of adenylate kinase, detection of cell proliferation antibodies
• Apoptosis assays using Annexin V detection.