Multi-Detection Microplate Reader

The Synergy HT Multi-detection reader is a robotic-compatible microplate reader that can measure absorbance, fluorescence, and luminescence. Its compact design and robot- friendly plate carrier make it an ideal platform for HTS and drug discovery environments. The Synergy HT utilizes a unique dual-optics design . It has both a monochromator/xenon flash system with a silicone diode detector for absorbance, and a tungsten halogen lamp with blocking interference filters and a PMT detector for fluorescence.

The absorbance optics are in a single-channel system that has the capability of measuring absorbance from the UV to the near IR range in all plate formats up to 384-well microplates. The monochromator based absorbance optics have a wavelength range of 200-999 nm, require no absorbance filters, and can perform spectral scans of substances in increments as small as 1 nm. A xenon-flash lamp is used to illuminate a high-precision diffraction-grating monochromator. The monochromatic light is split into an experimental and a reference channel. The experimental channel is next focused onto the microplate well sample, while the reference channel is directed to the reference detector. The unabsorbed light is then focused onto the silicone diode detector. By utilizing the near IR capability of the instrument, sample pathlength can be corrected automatically in liquid samples. This allows for the direct quantitation of unknown sample concentrations from absorbance measurements using extinction coefficients of the analyte rather than a standard curve.

• Detection method: Fluorescence, Time Resolved Fluorescence, UV-Visible Absorbance Luminescence
• Detection method: End-point, kinetic, spectral scanning, well-area scanning
• Microplate types: 6- to 1536-well plates (luminescence 1-384); PCR plates; Compatible with Take3 Multi-Volume Plate with 2 µL microspots
• Temperature control: 4°C above ambient to 65°C
• Shaking: Included
• PC Software Gen5™
• Control through USB or serial port
• Reagent Delivery: 10-300 microletres in 1 in microletre increments
• CV Precision: less than 0.7 percent at 300 ul
• Sample Delivery: Pipetted Probe Increments 0.1 microlitre increments from 1-100 microletres
• System Throughput (Clinical Chemistry): 190 test/hr- 280 using (ISE) test/hr
• Coagulation Mode: 70 PTs /hr
• Photometric Resolution: 0.001 O.D.

High Throughput Screening