The Pyrosequencer is used to detect and quantify genetic variations within short, targeted DNA sequences. It can detect low-level variation in multiple samples quickly, making it cost effective and efficient. The biotinylated single-stranded DNA template is loaded on a plate, and the machine adds dNTPs. The dNTPs release pyrophosphate when attached to the template, allowing for a signal to be given off in respect to which dNTP and the quantity that is incorporated.
- Input voltage and current:100–240 V AC, 47–63 Hz, 1.1–0.45 A (grounded) From the external power supplies to the instrument: 12 VDC and 24 VDC nominal - Power consumption Maximum: 160 Watt - Laboratory environment: Ambient temperature: 15–32°C (59–90°F) - Ambient humidity: 20–90% relative humidity - Weight: 27.5 kg (60.6 lb.) - Connections: One USB port (2.0) - Capacity:1–24 samples/run - Batch format: 24-well microtiter plate, PyroMark Q24 Plate - Process temperature: 28°C (82.4°F) +/-1% - Process time: Depending on the number of dispensations (20 dispensations take 24 minutes) - PyroMark Q24 Vacuum Workstation - Laboratory environment: Ambient temperature: 15–32°C (59–90°F) - Ambient humidity: 20–90% relative humidity - Process time: Less than 13 minutes for up to 24 samples in parallel
- Mutation analysis: Pyrosequencing technology is based on the sequencing by synthesis principle. Incorporation of a nucleotide by a polymerase using a single-stranded PCR (or RT-PCR) fragment as template ensures precision and accuracy when performing highly sensitive mutational analysis.
- Methylation analysis: Pyrosequencing generates highly reproducible quantification of methylation frequencies at individual consecutive CpG sites. Built-in bisulfite treatment controls eliminate manual estimation of non-converted DNA levels and prevent false-positive methylation detection, ensuring reliable results.
- Microbial identification: Unlike hybridization techniques, Pyrosequencing allows the identification of a large number of species using a single conserved primer. Consequently, DNA extracted from multiple microbe species can be sequenced in the same pyrosequencing run. PyroMark IdentiFire Software compiles local sequence database against which imported
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