Luminex 200 Multiplexing Instrument with Milliplex Analyst 5.1 software (Millipore)

The Luminex® 100/200™ System is a flexible analyser based on the principles of flow cytometry. The system enables you to multiplex (simultaneously measure) up to 100 analytes in a single microplate well, using very small sample volumes. The system delivers fast and cost-effective bioassay results on many assay formats including nucleic acid, receptor-ligand, immunoassays, and enzymatic assays. The Luminex 100/200 System is the combination of 3 core xMAP® Technologies. The first is xMAP microspheres, a family of fluorescently dyed micron-sized polystyrene microspheres that act as both the identifier and the solid surface to build the assay. The second is a flow cytometry-based instrument, the Luminex 100/200 analyser, which integrates key xMAP detection components, such as lasers, optics, fluidics, and high-speed digital signal processors. The third component is the xPONENT® software, which is designed for protocol-based data acquisition with robust data regression analysis.

• Capture Sandwich Immunoassay for replacing ELISAs with multiplexing and getting more information from a smaller sample
• Competitive Immunoassay enables detection of an anti- gen (target) with use of a single capture antibody attached to the surface of a microsphere and a competitive, labelled antigen reversibly bound to the antibody
• Serology Assay measures antibodies in experimental samples
• Gene Expression for moderate to high multiplexing (be- yond 100-plex) and high-throughput with robust reproducibility
• Genotyping for examining SNPs with several different methods
• MicroRNA Expression for detection of miRNA targets of interest that can be quantified in less than five hours